KMID : 1098420090170060434
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Korean Journal of Medicinal Crop Science 2009 Volume.17 No. 6 p.434 ~ p.438
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Optimal Extraction Condition and Characterization Antidementia Acetylcholinesterase Inhibitor from Job`s Tears(Coix Lachrymajobi L.)
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Seo Dong-Soo
Jang Jeong-Hoon Kim Na-Mi Lee Jong-Soo
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Abstract
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For the development of a new antidementia functional food or alternative drug using agricultural products, Job¡¯s tears (Coix lachrymajobi L.), which shows high acetylcholinesterase (AChE) inhibitory activity (55.1%) was selected and the extraction conditions of AChE inhibitor were optimized. AChE inhibitor of Job¡¯s tears was maximally extracted when it was treated with 60% methanol at 40¡É for 6 h. The AChE inhibitor of the methanol extracts was partially purified by systematic solvent extraction, thin layer chromatography, silica gel chromatography and reverse-phase HPLC and the partial purified AChE inhibitor with inhibitory activity (IC50) of 0.608 §¶ was obtained. The partial purified AChE inhibitor was soluble in methanol and hexane, and insoluble in water. Its maximum absorption spectra was 230 §¬ and also it was stable in the range of 30¡É and 70¡É and pH 4.0-8.0 for 1 h.
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KEYWORD
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Antidementia, Acetylcholinesterase Inhibitor, Job`s Tears
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